Name

HiFiScript 1st Strand cDNA Synthesis Kit

Cat. #

Application

• Reverse transcription, 1st strand cDNA synthesis
• cDNA library construction

Specifications

HiFiScript 1st Strand cDNA Synthesis Kit has been designed for the first-strand cDNA synthesis kit in a two-step RT-PCR experiments. HiFiScript reverse transcriptase in the kit is a new recombinant Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) with novel mutations, which endorse HiFiScript the following features:

• High transcriptional efficiency – higher yields of cDNA
• High sensitivity – pg level total RNA and/or mRNA can be used to synthesis first strand cDNA.
• RNase H activity is removed – improved the elongation ability of the enzyme, and increased affinity of transcriptase to RNA template. Generate first strand cDNA up to 12 kb.

The optimized RT Buffer make the reverse transcriptase a wider range of applications, and with high compatibility for subsequent PCR and quantitative PCR experiments.

Shipping / Storage

Manual (protocol)

RT reaction system

Note: If the starting RNA is less than 50 ng, RNase Inhibitor (RNasin, Cat.#: W0596) should be added.