Also available at      &   VWR
Mycoplasma Detection qPCR Kit (100 rxn)

Name

Mycoplasma Detection qPCR Kit (100 rxn)

Cat. #

T42030
$299.00
Product Size 100 reactions

Description

Mycoplasma contamination poses a significant threat to cell culture integrity, leading to substantial economic losses. Addressing this pressing issue, our team has developed a comprehensive range of solutions and reagents. From sample collection and DNA preparation to real-time PCR detection, our offerings ensure thorough mycoplasma detection and subsequent eradication. Customer feedback has been overwhelmingly positive, underscoring the effectiveness and reliability of our products in safeguarding cell cultures.

Mycoplasma Species qPCR Kit has been designed to specifically identify the mycoplasma genus species in a single PCR reaction using real-time quantitative polymerase chain reaction (qPCR) and probe fluorescence labels. The detection of the target DNA confirms ingredient authenticity and prevents food fraud, ethical issues, or health concerns. Our Mycoplasma qPCR is very sensitive, accurate, and highly efficient. (See Fig.1 and Table 1)

Applications

Detect Mycoplasma target DNA in contaminated cell cultures, FBS medium, plant, cannabis, cannabis ingredients, grain, food, herbals, and animal feed.

Feedback from users

DNA extraction protocol was simple and efficient, only requiring enrichment and boiling steps to achieve sufficient amounts of Mycoplasma DNA. The other kit components for RT-qPCR also worked well with no errors.
User friendly – highly recommended.

Features

High sensitivity and specificity for Mycoplasma species.
High efficiency: the optimal systemic conditions for PCR amplifications.
Streamlined protocol: Just add DNA Template and water.
No cross reactivity with other species

Shipping / Storage

Ship at 4℃,Store at -20℃ for up to 12 months and avoid freeze-thaw cycles.

Manual (protocol)

Detection Species Sheet:

Fig.1. Mycoplasma DNA concentration dependent qPCR Amplification (FAM)

Fig.2 Internal Control Gene qPCR Amplification (Hex)

Fig.3. Mycoplasma DNA preparation method validation

Table 1: Mycoplasma DNA qPCR Sensitivity

Table 2: Mycoplasma DNA efficiency comparison from 3 methods

Customer reviews for Mycoplasma Detection qPCR Kit.

- "User-friendly, highly recommended!"

- "Excellent products! The data is precise, and the manual is clearly written."

- "DNA extraction protocol was simple and efficient, only requiring enrichment and boiling steps to achieve sufficient amounts of Mycoplasma DNA. The other kit components for RT-qPCR also worked well with no errors."

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