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Mycoplasma Detection qPCR Kit


Mycoplasma Detection qPCR Kit

Cat. #


Product Size

100 reactions


Mycoplasma Species qPCR Kit has been designed to specifically identify the mycoplasma genus species in a single PCR reaction using real-time quantitative polymerase chain reaction (qPCR) and probe fluorescence labels. The detection of the target DNA confirms ingredient authenticity and prevents food fraud, ethical issues, or health concerns. Tribioscience’s Mycoplasma qPCR is very sensitive, accurate, and High efficiency (See Fig.1 and Table 1).


Detect Mycoplasma target DNA in contaminated cell cultures, FBS medium, plant, cannabis, cannabis ingredients, grain, food, herbals, and animal feed.

Feedback from users

DNA extraction protocol was simple and efficient, only requiring enrichment and boiling steps to achieve sufficient amounts of Mycoplasma DNA. The other kit components for RT-qPCR also worked well with no errors.
User friendly – highly recommended.


High sensitivity and specificity for Mycoplasma species.
High efficiency: the optimal systemic conditions for PCR amplifications.
Streamlined protocol: Just add DNA Template and water.
No cross reactivity with other species

Shipping / Storage

Ship at 4℃,Store at -20℃ for up to 12 months and avoid freeze-thaw cycles.

Manual (protocol)

Detection Species Sheet:

Fig.1. Mycoplasma DNA concentration dependent qPCR Amplification (FAM)

Fig.2 Internal Control Gene qPCR Amplification (Hex)

Fig.3. Mycoplasma DNA preparation method validation

Table 1: Mycoplasma DNA qPCR Sensitivity

Table 2: Mycoplasma DNA efficiency comparison from 3 methods

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