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Endotoxin-Free Plasmid Miniprep Kit


Endotoxin-Free Plasmid Miniprep Kit

Cat. #

(50 rxn)            order from  Fisher (Fisher Cat.# NC1492021)
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Sample Yield Elution Method DNA Quality
1 ~ 5mL E. Coli 3 ~ 30 μg EndoFree plasmid DNA 50 ~ 100 μL Spin Column Transfection, PCR, Enzyme digestion, Sequencing
This product is for research use only.

Description and features

Eukaryotic cells are very sensitive to endotoxin, a common contaminant in the plasmid extraction, and the transfection efficiency of the cells will greatly reduce if the plasmid contains endotoxin. This kit can efficiently extract endotoxin-free plasmid in a fast and simple way. The endotoxin, genomic DNA, RNA, protein and other impurities can be largely removed.

3 ~30 μg transfection-grade plasmid DNA can be purified from 1 ~ 5 mL overnight culture of E. Coli in LB medium with this kit within 30 minutes.

The extracted DNA can be directly used for Transfection PCR, restriction enzyme digestion, sequencing, etc. No need to precipitate, concentrate or desalt.

Shipping / Storage

Ship at room temperature. Store RNase A or RNase A added Buffer P1 at 4℃

Shelf Life

12 months

Manual (protocol)
  Endotoxin-Free Plasmid Miniprep Kit

General Procedure

Endotoxin-Free Plasmid DNA Miniprep Kit, EndoFree Plasmid DNA purification Kit


Components Amount Storage
Buffer P1 15 mL Room temperature; store at 4℃ if RNase A is added.
Buffer P2 15 mL Room temperature
Buffer E3 15 mL Room temperature
Buffer PS 15 mL Room temperature
Buffer PW ( concentrate ) 10 mL Room temperature
Endotoxin-Free Buffer EB 10 mL Room temperature
RNase A ( 10 mg/mL ) 150 μL 4oC
Endotoxin-Remover Filter with collection tube 50 sets Room temperature
Spin Column (Mini) with collection tube 50 sets Room temperature
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