1-Step 2X Super Multiplex RT-PCR Master Mix-TaqMan ProbeMake RNA analysis as easy as DNA analysis.
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1-Step 2X Super Multiplex RT-PCR Master Mix-TaqMan Probe |
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Application |
Make RNA analysis as easy as DNA analysis.
- The 1-Step 2X Super Multiplex RT-PCR Master Mix is used for real-time qualitative and quantitative multiplex RT-PCR amplifications with TaqMan probes. - This kit is designed for multiplex RT-PCR with up to five pairs of primers and five TaqMan probes. - The master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probes and RNA templates. - In a single-tube format, multiplex RNA templates are first converted to cDNAs by a reverse transcriptase, then a DNA-dependent DNA polymerase amplifies the cDNAs and its 5´-3´exonuclease cleaves TaqMan probes to generate fluorescent signals. - For the reverse transcription step, this kit uses a super-efficient Thermophilic Reverse Transcriptase (US patent pending), a thermophilic type A polymerase, which works best at 60-62°C, overcoming difficulties due to high GC content and abundant secondary structure. The RTase efficiently synthesizes a complementary DNA strands on RNA templates with 1-2 unit per 20μL of reaction. The RTase reversely-transcribes single digit copies of target RNA molecules consistently. - This kit also contains Super Taq-Probe DNA polymerase, which is specially engineered for TaqMan probes and its increased 5’-3’ exonuclease activity generating S-shaped curves. |
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Features |
- For the reverse transcription step, this kit uses a super-efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C.
- The RTase is easily heat-inactivated at ≥90°C for 1min. - The RTase efficiently synthesizes complementary DNA strands on RNA templates from agene-specific primers, 1-2 unit per 20μL of reaction. - The RTase reversely-transcribes single digit copies of target RNA molecules consistently. - This kit also contains Super Taq-Probe DNA polymerase specially engineered for TaqMan probes, generating S-shaped curves. - The preferable PCR product sizes are ≤150bp. - It is formulated as No ROX, Low ROX or High ROX concentrations for your choice. |
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Manual (protocol) |
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Q&A |
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