Also available at      &   VWR
1-Step 2X Multiplex RT-PCR Master Mix-TaqMan ProbeMake RNA analysis as easy as DNA analysis.


1-Step 2X Multiplex RT-PCR Master Mix-TaqMan Probe

Buy from


Make RNA analysis as easy as DNA analysis.
- The 1-Step 2X Multiplex RT-PCR Master Mix is used for qualitative and quantitative multiplex RT-PCR amplifications with TaqMan probes for up to 4 targets.
- The master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probes and RNA templates.


- For the reverse transcription step, this kit uses a highly efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase with optimal temperatures of 60-62°C, and it is heat-inactivated at ≥90°C.
- The RTase efficiently synthesizes complementary DNA strands on RNA templates from gene-specific primers, ≥1 unit per 20μL of multiplex reaction.
- The RTase can detect single digit copies of target RNA molecules.
- This kit also contains Taq-Probe DNA polymerase for the amplification step, specially engineered for TaqMan probes and generating S-Shaped curves.
- Two–four pairs of gene-specific primers can be applied in one reaction.
- The preferable PCR product sizes are ≤150bp.


Mouse Tail DNA Extraction only 20 minutes
3D Cell Culture Gel 30% < Mkt Price
PCR Kits 50% < Mkt price
Beta-Hexosaminidase Activity Colorimetric Assay Fast and sensitive, High-throughput
Endotoxin-Free Plasmid Kits maxi, midi and mini-prep