Also available at      &   VWR
Pure Plasmid Miniprep Kit


Pure Plasmid Miniprep Kit

Cat. #

$  299.00
(  50 reactions)


Sample Yield Elution Method DNA Quality
1 ~ 5 mL E. Coli 3 ~ 30 μg plasmid DNA 50 ~ 100 μL Spin Column Transfection, PCR, Enzyme digestion, Sequencing
This product is for research use only.

Description and features

This kit is based on a modified alkaline lysis method, with improved reagent formulation and silica membrane, which has high adsorption efficiency of the plasmid DNA. The proteins, genomes, RNA and other impurities can be removed after washing and an elution.

3 ~ 30 μg of plasmid DNA can be purified from 1 ~ 5 mL overnight culture of E. Coli in LB medium with this kit.

The extracted DNA can be directly used for PCR, restriction enzyme digestion, sequencing, and transfection. No need to precipitate, concentrate or desalt.

Shipping / Storage

Ship at room temperature. Store RNase A or RNase A added buffer P1 at 4℃

Shelf Life

12 months

Manual (protocol)
  Pure Plasmid Miniprep Kit

General Procedure

Pure Plasmid DNA Miniprep Kit, Plasmid DNA purification Kit


Components Amount Storage
Buffer P1 15 mL Room temperature; store at 4℃ if RNase A is added.
Buffer P2 15 mL Room temperature
Buffer N3 20 mL Room temperature
Buffer PB 30 mL Room temperature
Buffer PW ( concentrate ) 10 mL Room temperature
Buffer EB 10 mL Room temperature
RNase A ( 10 mg/mL ) 150 μL 4oC
Spin Column 50 Room temperature
2 mL Collection Tube 50 Room temperature
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