Pure Plasmid Miniprep Kit

Name

Pure Plasmid Miniprep Kit

Cat. #

W0500-50

$ 299.00

( 50 reactions)

How to pay with

Application

Sample	Yield	Elution	Method	DNA Quality
1 ~ 5 mL E. Coli	3 ~ 30 μg plasmid DNA	50 ~ 100 μL	Spin Column	Transfection, PCR, Enzyme digestion, Sequencing

This product is for research use only.

Description and features

This kit is based on a modified alkaline lysis method, with improved reagent formulation and silica membrane, which has high adsorption efficiency of the plasmid DNA. The proteins, genomes, RNA and other impurities can be removed after washing and an elution.

3 ~ 30 μg of plasmid DNA can be purified from 1 ~ 5 mL overnight culture of E. Coli in LB medium with this kit.

The extracted DNA can be directly used for PCR, restriction enzyme digestion, sequencing, and transfection. No need to precipitate, concentrate or desalt.

Shipping / Storage

Ship at room temperature. Store RNase A or RNase A added buffer P1 at 4℃

Shelf Life

12 months

Manual (protocol)

101Bio.com
Pure Plasmid Miniprep Kit

General Procedure

Pure Plasmid DNA Miniprep Kit, Plasmid DNA purification Kit

Components

Components	Amount	Storage
	W0500-50
Buffer P1	15 mL	Room temperature; store at 4℃ if RNase A is added.
Buffer P2	15 mL	Room temperature
Buffer N3	20 mL	Room temperature
Buffer PB	30 mL	Room temperature
Buffer PW ( concentrate )	10 mL	Room temperature
Buffer EB	10 mL	Room temperature
RNase A ( 10 mg/mL )	150 μL	4^oC
Spin Column	50	Room temperature
2 mL Collection Tube	50	Room temperature

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Name

Pure Plasmid Miniprep Kit

Cat. #

Step 1

Step 2

Application

Description and features

Shipping / Storage

Shelf Life

Manual (protocol)

General Procedure

Components