Name

Tryptase Activity Assay

Cat. #

Product Size

Description and features

Tryptase Activity Assay Kit provides a quick, efficient, and sensitive system for evaluating tryptase activity in cell lysates and supernatants and can be used in inhibitor screening.

Tryptase, a tetrameric serine proteinase, has emerged as the major component of mast cell granules. Tryptase comprises up to 20% of the total protein in mast cells derived from lung, colon, and skin tissue. Because it is stored almost exclusively in mast cells, tryptase is a popular indicator of mast cell activation and a target for therapeutic intervention in allergic diseases.

Applications

• • Cell culture supernatants, cell lysates, or other tryptase containing supplies.
• • Testing of purified tryptase enzyme, in vitro inhibitor screening.
• • Cell degranulation

Key Features

• • Sensitive and Accurate: The kit detects as low as 10μM in solution.
• • Simple and High-Throughput: The procedure is easily adapted to automation with no separation required as a high-throughput assay.

Manual (protocol)