Description : SPG67-1K / SPB67-1K: 2x PCR PreMix, with (green / blue) dye.

With non-interfering dye (green or blue), they are ready-to-use 2x concentrated solution for genotyping. Except DNA template and primers, the PCR master mix contains all other components for PCR, including Taq DNA polymerase, dNTPs, MgCl2, reaction buffer, PCR stabilizer and enhancer at optimal concentrations for consistent and efficient PCR amplification. For a 20 ul PCR reaction, simply add 10 ul of 2x PCR PreMix to specific primers, DNA template, and water. No additional loading buffer is required for electrophoresis when the PCR reaction is completed.
Optimal template and primer concentrations, and PCR program parameters should be determined experimentally by the investigator.

Features of the PCR master mix for genotyping :

* The PCR master mix simplifies the assembly of PCR reaction and offers advantages of time savings, convenience, consistency, and minimal risk of contamination and pipetting errors.
* The tracking dye and precipitant have been added into the PreMix so that the PCR product can be directly loaded for electrophoresis.
* Low cost: below the market prices.

Applications of the PCR master mix :

* Routine PCR using genomic, viral and plasmid templates.
* RT-PCR.
* DNA cloning and subcloning.
* Primer extension.
* DNA and colony screening.
* Genotyping.
* DNA mutagenesis.
* Generating hybridization probes for Southern or Northern hybridization.

The Taq DNA polymerase contained in the 2X PCR PreMix, with dye is available alone.

Storage/Stability: Stable for 1 year at -20°C and 2 weeks at 4°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.

Manual: the manual will be included in product bag.