Name |
FastSYBR Mixture |
Cat. # |
W0955-10
$ 289.00
W0955-25
$ 649.00
|
Product Description |
The FastSYBR Mixture is a premixed system for real-time fluorescence quantitative PCR (SYBR Green I), and the concentration is 2×. It contains Fast Taq DNA Polymerase, PCR Buffer, dNTPs, SYBR Green I Fluorescent Dye, and Mg2+. The operation is simple and convenient. This product is mainly used for the detection of genomic DNA target sequences and cDNA target sequences after RNA reverse transcription. This product contains the fluorescent dye SYBR Green I which can bind with all double-strand DNA, so that the product can be used for the detection of different target sequences without the need for the synthesis of specific labeled probes. The Fast Taq DNA Polymerase in the mixture is a chemically-modified, new efficient enzyme which reduce non-specific amplification efficiently, and it is activated by incubation at 95°C for 20 seconds, which will efficiently reduce the total reaction time. This product is suitable for fluorescent qPCR instruments with no ROX. This product is for research use only. No ROX as a calibration dye: Roche LightCycler 480, Roche LightCyler 96, Bio-rad iCyler iQ, iQ5, and CFX96. Need Low ROX (30-50mM) as a calibration dye: ABI Prism7500/7500 Fast, QuantStudio® 3 System, QuantStudio® 5 System, QuantStudio® 6 Flex System, QuantStudio® 7 Flex System, ViiA 7 system, Stratagene Mx3000/Mx3005P, Corbett Rotor Gene 3000 Need High ROX (300-500mM) as a calibration dye: ABI Prism7000/7300/7700/7900，Eppendorf，ABI Step One/Step One Plus. |
Shipping / Storage |
Store at -20°C, if used frequently, store at 2-8°C to avoid repeated freezing and thawing. Ship at 4°C. |
Manual (protocol) |
 |
FastSYBR Mixture
