Description

Cat. # SQP134: Universal SYBR Green qPCR Master Mix, Antibody-Based

The universal SYBR Green qPCR master mix, antibody-based is ready-to-use 2x concentrated solution for quantitative PCR by the SYBR Green I chimeric fluorescence method. Except DNA template and primers, the 2x universal SYBR Green qPCR master mix, antibody-based contains all other components for qPCR, including dNTP/dUTP mix, MgCl2, reaction buffer, antibody-based hot start Taq DNA polymerase at optimal concentrations for consistent and efficient PCR amplification. For a 20 ul reaction, simply add 10 ul of 2x universal SYBR Green qPCR master mix, antibody-based to specific primers, DNA template, and water.

Using the Taq DNA polymerase specific antibodies, the universal SYBR Green qPCR master mix gains the advantages of high specificity, high detection sensitivity, and high amplification yield. Combined with the optimal buffer optimized for qPCR and the specific promoting factors, the universal SYBR Green qPCR master mix greatly improves the qPCR amplification yield, with an ultra-high plateau value, and is suitable for qPCR amplification with high specificity and sensitivity. The universal SYBR Green qPCR master mix, antibody-based contains a unique ROX Reference Dye that is suitable for all qPCR instruments. The concentration of ROX does not need to be adjusted on different instruments.

Optimal template and primer concentrations, and qPCR program parameters should be determined experimentally by the investigator.

Features of the Universal SYBR Green qPCR Master Mix, Antibody-Based:
* High detection sensitivity.
* More stable amplification curve with low concentration template.
* Reduced formation of primer dimers and improved specificity of qPCR products.
* Convenient, easy, and quick operation.

Cat. # SQP134 Components of the Universal SYBR Green qPCR Master Mix, Antibody-Based:
2x universal SYBR Green qPCR master mix: Mixture of antibody-based hot start Taq DNA polymerase, dNTP mix, MgCl2, specific enhancer factors, SYBR Green I, and specific ROX reference dye.

Storage/Stability: Stable for 1 year at -70°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.

Cat. # SQP135 Components of the Universal SYBR Green qPCR Master Mix II, Antibody-Based:
2x universal SYBR Green qPCR master mix II: Mixture of antibody-based hot start Taq DNA polymerase II, dNTP mix, MgCl2, specific enhancer factors, SYBR Green I, and specific ROX reference dye.

Cat. # SQP136 Components of the SYBR Green qPCR Master Mix, Antibody-Based, with High ROX Premixed and Color Indication:
2x SYBR Green qPCR master mix: Mixture of antibody-based hot start Taq DNA polymerase, dNTP mix, MgCl2, specific enhancer factors, SYBR Green I, high concentration of ROX passive reference dye, and blue dye.
10x template dilution buffer: Yellow.

Cat. # SQP131 Components of the SYBR Green qPCR Master Mix, Antibody-Based, with Low ROX Premixed and Color Indication :
2x SYBR Green qPCR master mix: Mixture of antibody-based hot start Taq DNA polymerase, dNTP mix, MgCl2, specific enhancer factors, SYBR Green I, low concentration of ROX passive reference dye, and blue dye.
10x template dilution buffer: Yellow.

Cat. # SQP132 Components of the SYBR Green qPCR Master Mix, Antibody-Based, without ROX and Color Indication :
2x SYBR Green qPCR master mix: Mixture of antibody-based hot start Taq DNA polymerase, dNTP mix, MgCl2, specific enhancer factors, SYBR Green I, and blue dye.
10x template dilution buffer: Yellow.

Cat. # SQP133 Components of the SYBR Green qPCR Master Mix, Antibody-Based, with Color Indication:
2x SYBR Green qPCR master mix: Mixture of antibody-based hot start Taq DNA polymerase, dNTP mix, MgCl2, specific enhancer factors, SYBR Green I, and blue dye.
10x template dilution buffer: Yellow.
50x ROX reference dye 1.
50x ROX reference dye 2.

Cat. # SQP137: Universal SYBR Green qPCR Master Mix, Chemical-Based
The universal SYBR Green qPCR master mix, chemical-based is ready-to-use 2x concentrated solution for quantitative PCR by the SYBR Green I chimeric fluorescence method. Except DNA template and primers, the 2x universal SYBR Green qPCR master mix, chemical-based contains all other components for qPCR, including dNTP/dUTP mix, MgCl2, reaction buffer, chemically modified hot start Taq DNA polymerase at optimal concentrations for consistent and efficient PCR amplification. For a 20 ul reaction, simply add 10 ul of 2x universal SYBR Green qPCR master mix, chemical-based to specific primers, DNA template, and water.
Using the chemically modified Taq DNA polymerase, the universal SYBR Green qPCR master mix gains the advantages of high specificity, high sensitivity, and high amplification efficiency. Combined with the optimal buffer optimized for qPCR and the specific promoting factors, the universal SYBR Green qPCR master mix greatly improves the qPCR amplification yield, with a ultra-high plateau value, and is suitable for qPCR amplification with high specificity and sensitivity. The universal SYBR Green qPCR master mix, chemical-based contains a unique ROX Reference Dye that is suitable for all qPCR instruments. The concentration of ROX does not need to be adjusted on different instruments.
Optimal template and primer concentrations, and qPCR program parameters should be determined experimentally by the investigator.

Features of the Universal SYBR Green qPCR Master Mix, Chemical-Based:
* Universal on all platforms: Special ROX reference dye suitable for all qPCR instruments; no need to adjust ROX concentration on different qPCR instruments.
* Rigorous hot-start enzyme: Chemically modified Taq DNA Polymerase providing high amplification specificity.
* Optimized reaction buffer formula: Minimizing non-specific amplification and primer dimerization.
* High amplification performance: repeatable and reliable quantitative results.

Cat. # SQP137 Components of the Universal SYBR Green qPCR Master Mix, Chemical-Based:
2x universal SYBR Green qPCR master mix: Mixture of chemically modified hot start Taq DNA polymerase, dNTP mix, MgCl2, specific enhancer factors, SYBR Green I, and specific ROX reference dye.
Storage/Stability: Stable for 1 year at -70°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.

Cat. # SQP138 Components of the SYBR Green qPCR Master Mix, Chemical-Based, with High ROX Premixed:
2x SYBR Green qPCR master mix: Mixture of chemically modified hot start Taq DNA polymerase, dNTP mix, MgCl2, specific enhancer factors, SYBR Green I, and high concentration of ROX passive reference dye.

Cat. # SQP139 Components of the SYBR Green qPCR Master Mix, Chemical-Based, with Low ROX Premixed:
2x SYBR Green qPCR master mix: Mixture of chemically modified hot start Taq DNA polymerase, dNTP mix, MgCl2, specific enhancer factors, SYBR Green I, and low concentration of ROX passive reference dye.
Cat. # SQP140 Components of the SYBR Green qPCR Master Mix, Chemical-Based, without ROX:
2x SYBR Green qPCR master mix: Mixture of chemically modified hot start Taq DNA polymerase, dNTP mix, MgCl2, specific enhancer factors, and SYBR Green I.
Cat. # SQP141 Components of the SYBR Green qPCR Master Mix, Chemical-Based:
2x SYBR Green qPCR master mix: Mixture of chemically modified hot start Taq DNA polymerase, dNTP mix, MgCl2, specific enhancer factors, and SYBR Green I.
50x ROX reference dye 1.
50x ROX reference dye 2.