SQP148: Universal U+ Probe qPCR Master Mix, Antibody-Based
The universal U+ probe qPCR master mix, antibody-based is ready-to-use 2x concentrated solution for quantitative PCR by the probe method. Except DNA template and primers, the 2x universal U+ probe qPCR master mix, antibody-based contains all other components for qPCR, including dNTP/dUTP mix, MgCl2, reaction buffer, heat-labile UDG, and antibody-based hot start Taq DNA polymerase at optimal concentrations for consistent and efficient PCR amplification. For a 20 ul reaction, simply add 10 ul of 2x universal U+ probe qPCR master mix, antibody-based to specific primers, DNA template, and water.
Using the Taq DNA polymerase specific antibodies, the universal U+ probe qPCR master mix gains the advantages of high specificity, high detection sensitivity, and high amplification yield. Combined with the optimal buffer optimized for qPCR and the specific promoting factors, the universal U+ probe qPCR master mix greatly improves the qPCR amplification yield, with a ultra-high plateau value, and is suitable for qPCR amplification with high specificity and sensitivity. The universal U+ probe qPCR master mix, antibody-based contains a unique ROX Reference Dye that is suitable for all qPCR instruments. The concentration of ROX does not need to be adjusted on different instruments. The universal U+ probe qPCR master mix contains a dUTP/UDG system that can prevent the contamination of PCR product. Heat-labile UDG can quickly eliminate the contamination of dU-containing products at room temperature. It can be inactivated at 55℃ without affecting the efficiency and sensitivity of qRT-PCR.
Optimal template and primer concentrations, and qPCR program parameters should be determined experimentally by the investigator.

Features :
* High detection sensitivity.
* More stable amplification curve with low concentration template.
* Reduced formation of primer dimers and improved specificity of qPCR products.
* Convenient, easy, and quick operation.

# SQP148 Components of the Universal U+ Probe qPCR Master Mix, Antibody-Based:
2x universal U+ probe qPCR master mix: Mixture of antibody-based hot start Taq DNA polymerase, dNTP/dUTP mix, MgCl2, specific enhancer factors, heat-labile UDG, and specific ROX reference dye.
Storage/Stability: Stable for 1 year at -70°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.

# SQP149 Components of the Universal Probe qPCR Master Mix, Antibody-Based 2x universal probe qPCR master mix: Mixture of antibody-based hot start Taq DNA polymerase, dNTP mix, MgCl2, specific enhancer factors, and specific ROX reference dye.

# SQP150 Components of the U+ Probe qPCR Master Mix, Antibody-Based
2x U+ probe qPCR master mix: Mixture of antibody-based hot start Taq DNA polymerase, dNTP/dUTP mix, MgCl2, heat-labile UDG, and specific enhancer factors.
50x ROX reference dye 1.
50x ROX reference dye 2.

# SQP151 Components of the Probe qPCR Master Mix, Antibody-Based
2x probe qPCR master mix: Mixture of antibody-based hot start Taq DNA polymerase, dNTP mix, MgCl2, and specific enhancer factors.
50x ROX reference dye 1.
50x ROX reference dye 2.

# SQP155: Universal U+ Probe qPCR Master Mix, Chemical-Based
The universal U+ probe qPCR master mix, chemical-based is ready-to-use 2x concentrated solution for quantitative PCR by the probe method. Except DNA template and primers, the 2x universal U+ probe qPCR master mix, chemical-based contains all other components for qPCR, including dNTP/dUTP mix, MgCl2, reaction buffer, heat-labile UDG, and chemically modified hot start Taq DNA polymerase at optimal concentrations for consistent and efficient PCR amplification. For a 20 ul reaction, simply add 10 ul of 2x universal U+ probe qPCR master mix, chemical-based to specific primers, DNA template, and water.
Using the chemically modified Taq DNA polymerase, the universal U+ probe qPCR master mix gains the advantages of high specificity, high sensitivity, and high amplification efficiency. Combined with the optimal buffer optimized for qPCR and the specific promoting factors, the universal U+ probe qPCR master mix greatly improves the qPCR amplification yield, with a ultra-high plateau value, and is suitable for qPCR amplification with high specificity and sensitivity. The universal U+ probe qPCR master mix contains a dUTP/UDG system that can prevent the contamination of PCR products. Heat-labile UDG can quickly eliminate the contamination of dU-containing products at room temperature. It can be inactivated at 55℃ without affecting the efficiency and sensitivity of qRT-PCR. The universal U+ probe qPCR master mix, chemical-based contains a unique ROX Reference Dye that is suitable for all qPCR instruments. The concentration of ROX does not need to be adjusted on different instruments.
Optimal template and primer concentrations, and qPCR program parameters should be determined experimentally by the investigator.

# SQP155 Features of the Universal U+ Probe qPCR Master Mix, Chemical-Based:
* Universal on all platforms: Special ROX reference dye suitable for all qPCR instruments; no need to adjust ROX concentration on different qPCR instruments.
* Rigorous hot-start enzyme: Chemically modified Taq DNA Polymerase providing high amplification specificity.
* Optimized reaction buffer formula: Minimizing non-specific amplification and primer dimerization.
* High amplification performance: repeatable and reliable quantitative results.

# SQP155 Components of the Universal U+ Probe qPCR Master Mix, Chemical-Based:
2x universal U+ probe qPCR master mix: Mixture of chemically modified hot start Taq DNA polymerase, dNTP/dUTP mix, MgCl2, specific enhancer factors, heat-labile UDG, and specific ROX reference dye.
Storage/Stability: Stable for 1 year at -70°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.

# SQP156 Components of the U+ Probe qPCR Master Mix, Chemical-Based
2x U+ Probe qPCR master mix: Mixture of chemically modified hot start Taq DNA polymerase, dNTP/dUTP mix, MgCl2, heat-labile UDG, and specific enhancer factors.
50x ROX reference dye 1.
50x ROX reference dye 2.

SQP157 Components of the Probe qPCR Master Mix, Chemical-Based
2x probe qPCR master mix: Mixture of chemically modified hot start Taq DNA polymerase, dNTP mix, MgCl2, and specific enhancer factors.
50x ROX reference dye 1.
50x ROX reference dye 2.