Lentivirus Packaging Kit
Name |
Lentivirus Packaging Kit |
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101Bio Cat. #: P904
$579.00
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| Related Product : |
Lentivirus / Retrovirus Concentration Solution, P904C, 100 ml, Fisher Sci NC3242189 Lentivirus Packaging Mix, Cat.# P904P |
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Application |
For packaging Lentivirus. This product is for research use only. | ||||||||||||
Product Size |
10 reactions, producing Lentivirus in 100mm dish per reaction | ||||||||||||
Description |
This kit is for packaging 3rd generation Lentivirus. To package Lentivirus, use the provided transfection reagent to transfect 293T cells with your lentiviral vector together and the provided packaging mix. The packaging plasmids will express the elements required for lentivirus production. Replication-incompetent, HIV-1-based lentivirus containing your gene of interest will be produced. |
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Shipping / Storage |
Ship at 4℃, Store at 4℃ and -20℃ (see below) | ||||||||||||
Shelf Life |
6 months | ||||||||||||
Manual (protocol) |
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Component |
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Remarks |
The 3rdgeneration packaging system offers maximal biosafety as the lentiviral Rev gene is supplied as an independent vector from other structure genes, further eliminating the possibility of reverse recombination of vectors into a replication competent viral particle. The third generation lentiviral packaging mix will only support lentiviral expression vector with a chimeric 5' LTR in which the HIV promoter is replaced with CMV or RSV, thus making it TAT-independent. Follow the recommended NIH BSL-2 guidelines for all materials containing Lentivirus. |
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