Background

The MOPC-21 antibody (IgG1, kappa) has been used for a long time as an isotype-matched control antibody to estimate the degree of non-specific binding by an antigen-specific antibody. The variable regions of the MOPC-21 antibody share sequences with the antibodies against all members of the dengue virus complex, and the Epstein-Barr virus gp350 protein. The recombinant MOPC-21 IgG1 kappa isotype control and mutants (natural and engineered mIgG1 isotype controls) are useful for in vitro and in vivo studies from ELISA, Western blot (WB), to cell-based assay to animal model study. Mouse IgG1 isotype control and mutants (natural and engineered mIgG1 isotype controls) are useful for in vitro and in vivo studies from ELISA, Western blot (WB), to cell-based assay to animal model study. The isotypes of mouse IgG antibody reagents used in scientific research and clinical diagnostics are mainly mouse IgG1 kappa. Mouse IgG1 antibodies and engineered mutants are also used in therapeutic drug discovery. Mouse IgG1 antibodies are more like human IgG4 antibodies than human IgG1 antibodies, which behave more like mouse IgG2a or IgG2c antibodies. Many antibody drugs especially those targeting immune checkpoint proteins use the human IgG4 constant regions.

As an ideal recombinant mouse IgG1 isotype control, the recombinant MOPC-21 mIgG1 kappa isotype control has low or no specific binding to any mouse sample, and completely mouse variable regions and the mouse IgG1 kappa constant regions. The mouse IgG1 kappa isotype control and mutants can be used in ELISA, Western blot (WB), the cell-based assay or animal model as the antibody negative control.

Please contact us to ask for a quote for the engineered MOPC-21 mouse IgG1 isotype control mutants, mIgG1 isotype control and mutants with Avi-, His-, and Flag-tags, and biotinylated mIgG1 isotype control and mutants. A variety of conjugates (such as dyes and fluorophores) with the MOPC-21 mouse IgG1 isotype control and mutants are available.

SA155.m1: In Vivo Grade Recombinant MOPC-21 Mouse IgG1 Kappa Isotype Control Antibody (mIgG1 Isotype Control)

The in vivo grade recombinant MOPC-21 mouse IgG1 isotype control antibody (mIgG1 isotype control) was produced in mammalian cells.

Immunogen: N/A.
Clone: MOPC-21.
Isotype: mouse IgG1, kappa.
Applications: an isotype-matched negative control for mouse IgG1 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
Shipping: The in vivo grade recombinant MOPC-21 mouse IgG1 kappa isotype control antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.

In Vivo Grade Recombinant Isotype Control Antibodies (clone MOPC-21):
In vivo Grade Recombinant MOPC-21 Mouse IgG1 Isotype Control Antibody Mutants
In vivo Grade Recombinant MOPC-21 Mouse IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant MOPC-21 Mouse IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant MOPC-21 Mouse IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant MOPC-21 Mouse IgG3 Isotype Control Antibody and Mutants
In vivo Grade Recombinant MOPC-21 Mouse Fab Fragment
In vivo Grade Recombinant MOPC-21 Human IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant MOPC-21 Human IgG2 Isotype Control Antibody and Mutants
In vivo Grade Recombinant MOPC-21 Human IgG3 Isotype Control Antibody and Mutants
In vivo Grade Recombinant MOPC-21 Human IgG4-S228P Isotype Control Antibody and Mutants
In vivo Grade Recombinant MOPC-21 Human Fab Fragment
In vivo Grade Recombinant MOPC-21 Human IgE Isotype Control Antibody
In vivo Grade Recombinant MOPC-21 Human IgM Isotype Control Antibody
In vivo Grade Recombinant MOPC-21 Human IgA1 Isotype Control Antibody
In vivo Grade Recombinant MOPC-21 Rat IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant MOPC-21 Rat IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant MOPC-21 Rat IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant MOPC-21 Rat IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant MOPC-21 Rat Fab Fragment
In vivo Grade Recombinant MOPC-21 Hamster IgG1 Isotype Control Antibody
In vivo Grade Recombinant MOPC-21 Hamster IgG2 Isotype Control Antibody
In vivo Grade Recombinant MOPC-21 Rabbit IgG Isotype Control Antibody
In vivo Grade Recombinant MOPC-21 Ferret IgG1 Isotype Control Antibody
In vivo Grade Recombinant MOPC-21 Ferret IgM Isotype Control Antibody
In vivo Grade Recombinant MOPC-21 Ferret IgA Isotype Control Antibody
In vivo Grade Recombinant MOPC-21 Canine IgG1 Isotype Control Antibody
In vivo Grade Recombinant MOPC-21 Canine IgG2 Isotype Control Antibody
In vivo Grade Recombinant MOPC-21 Canine IgG3 Isotype Control Antibody
In vivo Grade Recombinant MOPC-21 Canine IgG4 Isotype Control Antibody
In vivo Grade Recombinant MOPC-21 Feline IgG1 Isotype Control Antibody